Research Paper Volume 13, Issue 1 pp 1017—1031

AIM2 inhibits colorectal cancer cell proliferation and migration through suppression of Gli1

AIM2 regulates Gli1-mediated EMT through AKT/mTOR pathway. (A) Western blots of indicated proteins in SW480 and SW620 cells stably transfected with control-shRNA (NC) or shRNA against AIM2 (KD). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. (B) Western blots of indicated proteins in HCT116 and LoVo cells stably transfected with empty vector (VEC) or plasmid encoding human AIM2 (AIM2). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. (C, D) Western blots of indicated proteins in SW480 (C) and SW620 (D) cells stably transfected with control-shRNA (NC) or shRNA against AIM2 (KD) with or without Ly294002 (20 μM). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. *P

Figure 6. AIM2 regulates Gli1-mediated EMT through AKT/mTOR pathway. (A) Western blots of indicated proteins in SW480 and SW620 cells stably transfected with control-shRNA (NC) or shRNA against AIM2 (KD). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. (B) Western blots of indicated proteins in HCT116 and LoVo cells stably transfected with empty vector (VEC) or plasmid encoding human AIM2 (AIM2). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. (C, D) Western blots of indicated proteins in SW480 (C) and SW620 (D) cells stably transfected with control-shRNA (NC) or shRNA against AIM2 (KD) with or without Ly294002 (20 μM). GAPDH as a loading control. Each experiment was performed at least triplicate and the bands were quantified and presented as the mean±SEM. *P<0.05, **P<0.01, based on a two-tailed paired Student’s t-test or ANOVA.