Research Paper Volume 13, Issue 1 pp 1096—1119

The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles

FBXW7 destabilized mTOR protein in a ubiquitin/proteasome-dependent manner. (A) The relative mRNA levels of FBXW7 in ovaries from 1-7 dpp. (B) The correlation between the mRNA levels of FBXW7 and the protein levels of mTOR. (C) Immunofluorescence analysis displaying the colocalization of mTOR and FBXW7 in neonatal ovaries. Arrows indicate PFs and triangles indicate GFs. Scale bar, 40 μm. (D) Immunoblotting analysis of Co-IP against mTOR. (E) Immunoblotting analysis of Co-IP against FBXW7. (F) Six days after transfection, immunofluorescence staining for Flag was performed in the empty vector- and FBXW7 overexpression (OE) vector-treated ovaries. Scale bar, 40 μm. (G) Three days after transfection, the relative mRNA levels of FBXW7 were detected in the empty vector and OE vector groups. (H) Western blotting analysis of mTOR protein levels in ovaries treated for 4 h with CHX or SN + CHX three days after transfection. (I) Immunoblotting analysis of Co-IP against mTOR in ovaries three days after transfection. ** and *** denote statistical significance at p p

Figure 7. FBXW7 destabilized mTOR protein in a ubiquitin/proteasome-dependent manner. (A) The relative mRNA levels of FBXW7 in ovaries from 1-7 dpp. (B) The correlation between the mRNA levels of FBXW7 and the protein levels of mTOR. (C) Immunofluorescence analysis displaying the colocalization of mTOR and FBXW7 in neonatal ovaries. Arrows indicate PFs and triangles indicate GFs. Scale bar, 40 μm. (D) Immunoblotting analysis of Co-IP against mTOR. (E) Immunoblotting analysis of Co-IP against FBXW7. (F) Six days after transfection, immunofluorescence staining for Flag was performed in the empty vector- and FBXW7 overexpression (OE) vector-treated ovaries. Scale bar, 40 μm. (G) Three days after transfection, the relative mRNA levels of FBXW7 were detected in the empty vector and OE vector groups. (H) Western blotting analysis of mTOR protein levels in ovaries treated for 4 h with CHX or SN + CHX three days after transfection. (I) Immunoblotting analysis of Co-IP against mTOR in ovaries three days after transfection. ** and *** denote statistical significance at p < 0.01 and p < 0.001, respectively.