Research Paper Volume 13, Issue 3 pp 3202—3217

GV1001 interacts with androgen receptor to inhibit prostate cell proliferation in benign prostatic hyperplasia by regulating expression of molecules related to epithelial-mesenchymal transition

Regulation of TGF-β production and EMT-related molecules, and alleviation of fibrosis, by GV1001 in the prostate of BPH mice. BPH mice were treated with GV1001 (GV) or finasteride (F) for 2 weeks. (A) After prostates were homogenized, the level of TGF-β in the lysates was measured in an ELISA. *pB) Expression of EMT-related proteins (Snail, Slug, N-cadherin, Vimentin, and E-cadherin) in the prostate was examined by immunofluorescence. Scale bar, 150 μm. (C) Prostates were fixed and sections were stained with Masson’s Trichrome. Scale bar, 100 μm. Asterisks (*) indicate smooth muscle (red). Collagen fibers were stained blue. The bar graph shows the area of fibrosis stained positive with Masson Trichrome. ***p

Figure 4. Regulation of TGF-β production and EMT-related molecules, and alleviation of fibrosis, by GV1001 in the prostate of BPH mice. BPH mice were treated with GV1001 (GV) or finasteride (F) for 2 weeks. (A) After prostates were homogenized, the level of TGF-β in the lysates was measured in an ELISA. *p<0.05. (B) Expression of EMT-related proteins (Snail, Slug, N-cadherin, Vimentin, and E-cadherin) in the prostate was examined by immunofluorescence. Scale bar, 150 μm. (C) Prostates were fixed and sections were stained with Masson’s Trichrome. Scale bar, 100 μm. Asterisks (*) indicate smooth muscle (red). Collagen fibers were stained blue. The bar graph shows the area of fibrosis stained positive with Masson Trichrome. ***p<0.001.