Research Paper Volume 12, Issue 24 pp 26029—26046

DUSP5 suppresses interleukin-1β-induced chondrocyte inflammation and ameliorates osteoarthritis in rats

Figure 3. Effect of DUSP5 knockdown on IL-1β-induced gene expression. (A) Levels of DUSP5, iNOS, COX2, MMP9, MMP13, and MMP3 mRNAs as measured by reverse transcription-quantitative polymerase chain reaction. (B, C) The protein expression and quantitation of iNOS, COX2, MMP9, MMP13, DUSP5, TIMP3, and IL-10. GAPDH was used as the control. DUSP5 knockdown chondrocytes were incubated with IL-1β (10 ng/mL) for 24 h. All data are expressed as mean±S.D. (n = 3). *p<0.05, **p<0.01.