Research Paper Volume 13, Issue 1 pp 1186—1211

MicroRNA-139-5p upregulation is associated with diabetic endothelial cell dysfunction by targeting c-jun

Diabetes induces upregulation of miR-139-5p expression in endothelial cells. (A) MiR-139-5p expression in endothelial colony-forming cells (ECFCs) isolated and cultured from peripheral blood (PB) of diabetic patients and healthy adults was detected by real-time PCR. (N=4) *P B) MiR-139-5p expression in human umbilical vein endothelial cells (HUVECs) after 7 days of high glucose or high osmotic culture was detected by real-time PCR. (N=3) *P C) MiR-139-5p expression in ECFCs from health PB after 7 days of high glucose or high osmotic culture was detected by real-time PCR. (N=3) *P D) MiR-139-5p expression in endothelial cells derived from the thoracic aorta of normal and diabetic rats was detected by real-time PCR. (N=6) *P E) MiR-139-5p expression in endothelial cells derived from the thoracic aorta of normal and diabetic nude mouse was detected by real-time PCR. (N=6) *P F) The expression of miR-139-5p in different clone cells. (N=3) *P

Figure 1. Diabetes induces upregulation of miR-139-5p expression in endothelial cells. (A) MiR-139-5p expression in endothelial colony-forming cells (ECFCs) isolated and cultured from peripheral blood (PB) of diabetic patients and healthy adults was detected by real-time PCR. (N=4) *P < .05 (B) MiR-139-5p expression in human umbilical vein endothelial cells (HUVECs) after 7 days of high glucose or high osmotic culture was detected by real-time PCR. (N=3) *P < .05 versus day 1 (C) MiR-139-5p expression in ECFCs from health PB after 7 days of high glucose or high osmotic culture was detected by real-time PCR. (N=3) *P < .05 versus day 1 (D) MiR-139-5p expression in endothelial cells derived from the thoracic aorta of normal and diabetic rats was detected by real-time PCR. (N=6) *P < .05 versus normal rats. (E) MiR-139-5p expression in endothelial cells derived from the thoracic aorta of normal and diabetic nude mouse was detected by real-time PCR. (N=6) *P < .05 versus normal nude mouse. (F) The expression of miR-139-5p in different clone cells. (N=3) *P < .05 versus high proliferation potential ECFCs. Data shown in the graphs represent mean ± standard deviation.