Research Paper Volume 13, Issue 1 pp 1186—1211

MicroRNA-139-5p upregulation is associated with diabetic endothelial cell dysfunction by targeting c-jun

MiR-139-5p regulates the expression of c-jun. (A) C-jun expression was detected by Western blot in ECFCs derived from diabetic patients and healthy subjects. (N=3) *P B) C-jun expression was detected by Western blot in ECs derived from the thoracic aorta of diabetic rats and normal rats. (N=3) *P C) C-jun expression was detected by Western blot in normal ECFCs transfected with a miR-139-5p mimic and in diabetic ECFCs transfected with a miR-139-5p inhibitor. (N=3) *P D) Dual Luciferase Reporter Assay used to assess whether c-jun was the target of miR-139-5p. (N=3) *P

Figure 4. MiR-139-5p regulates the expression of c-jun. (A) C-jun expression was detected by Western blot in ECFCs derived from diabetic patients and healthy subjects. (N=3) *P < .05 versus normal ECFCs. (B) C-jun expression was detected by Western blot in ECs derived from the thoracic aorta of diabetic rats and normal rats. (N=3) *P < .05 versus normal rats. (C) C-jun expression was detected by Western blot in normal ECFCs transfected with a miR-139-5p mimic and in diabetic ECFCs transfected with a miR-139-5p inhibitor. (N=3) *P < .05 versus normal or diabetic ECFCs. (D) Dual Luciferase Reporter Assay used to assess whether c-jun was the target of miR-139-5p. (N=3) *P < .05. Data shown in the graphs represent mean ± standard deviation.