Research Paper Volume 13, Issue 1 pp 1186—1211

MicroRNA-139-5p upregulation is associated with diabetic endothelial cell dysfunction by targeting c-jun

Effect of c-jun on ECFCs. (A) Scratch assay detected the migration ability of ECFC transfected with c-jun siRNA. (N=3). (B, C) CCK8 and cell counting detected the proliferative ability of ECFCs treated with c-jun siRNA or overexpress plasmid. The nucleus was dyed blue by Hoechst stain and the proliferating cells were dyed red by 5-ethynyl-2′-deoxyuridine. The photos were captured by a 40X fluorescence microscope. (N=3) *P D) In vitro tube formation experiments detected the angiogenesis ability of ECFCs with different treatment. The photos were captured by a 40X fluorescence microscope. (N=3) *P

Figure 5. Effect of c-jun on ECFCs. (A) Scratch assay detected the migration ability of ECFC transfected with c-jun siRNA. (N=3). (B, C) CCK8 and cell counting detected the proliferative ability of ECFCs treated with c-jun siRNA or overexpress plasmid. The nucleus was dyed blue by Hoechst stain and the proliferating cells were dyed red by 5-ethynyl-2′-deoxyuridine. The photos were captured by a 40X fluorescence microscope. (N=3) *P < .05 versus control. (D) In vitro tube formation experiments detected the angiogenesis ability of ECFCs with different treatment. The photos were captured by a 40X fluorescence microscope. (N=3) *P < .05 versus control. Data shown in the graphs represent mean ± standard deviation.