Figure 4. Microglial autophagy was mitigated by PTP1B inhibitor treatment after IR injury. (A) Western blot to detect the effect of PTP1B inhibitor (10 μM) on LC3-I/II and beclin-1 protein expression in the rat ipsilateral cortex 24 h after IR injury. (B, C) Quantitative results of relative band density are normalized to β-actin and presented as the mean ± SEM (n = 4 per group). (D) Immunofluorescence to detect GFP-LC3B puncta in GFP-LC3 expressing BV-2 cells. Scale bar = 200 μm. (E) Graph showing percentage of cells with GFP-LC3 puncta (presented as the mean ± SEM, n = 6 per group). *p < 0.05; **p < 0.01; ***p < 0.001 compared with vehicle group; sc = PTP1B inhibitor sc-222227; IR = ischemia/reperfusion; OGD/R = oxygen glucose deprivation/reoxygenation.