Research Paper Volume 13, Issue 3 pp 3443—3458

Hypoxia-inducible factor 2-alpha-dependent induction of IL-6 protects the heart from ischemia/reperfusion injury

The effect of in vivo HIF2α knockdown on myocardial infarction during acute myocardial ischemia/reperfusion. (A) Myocardial I/R model was established 4 weeks after AAV injection. (B) Western blots were performed to examine the HIF2α and IL-6 protein expression after HIF2α knockdown in vivo. n = 3 per group. (C) TTC and Evans blue staining after mouse heart I/R. n = 8 per group. (D) Effect of HIF2α knockdown on myocardial LDH release subsequent to I/R. n = 5 per group. (E) Effect of HIF2α knockdown on myocardial TnI release subsequent to I/R. n = 5 per group. Data represent the mean ± SEM. **P

Figure 3. The effect of in vivo HIF2α knockdown on myocardial infarction during acute myocardial ischemia/reperfusion. (A) Myocardial I/R model was established 4 weeks after AAV injection. (B) Western blots were performed to examine the HIF2α and IL-6 protein expression after HIF2α knockdown in vivo. n = 3 per group. (C) TTC and Evans blue staining after mouse heart I/R. n = 8 per group. (D) Effect of HIF2α knockdown on myocardial LDH release subsequent to I/R. n = 5 per group. (E) Effect of HIF2α knockdown on myocardial TnI release subsequent to I/R. n = 5 per group. Data represent the mean ± SEM. **P<0.01 versus the indicated group.