Figure 1. Effects of DCM on Sirt1 expression and AMPK phosphorylation in older patients. Expression analysis of Sirt1 mRNA (A), total AMPK (B) and phosphorylated AMPK (Thr172) (C) performed with human cardiac tissue lysates from old control (non-diseased) and DCM men (♂) and women (♀). Data are means ± SEM (n= 5). Representative imaging of western blot analysis; the lanes were run in two gels. All data were normalized to the corresponding control and expressed in relative units (r.u.). (D) Linear regression analysis between pAMPK/AMPK ratio (dependent variable) and age (explanatory variable) was performed with function lm() in R. pAMPK/AMPK values were obtained from old and young male and female hearts diagnosed with DCM.