Research Paper Volume 12, Issue 24 pp 26080—26094

Postprandial triglyceride-rich lipoproteins-induced premature senescence of adipose-derived mesenchymal stem cells via the SIRT1/p53/Ac-p53/p21 axis through oxidative mechanism

Figure 1. Postprandial TRL induced both adipogenesis and premature senescence in AMSCs. (A) AMSCs were treated with PBS, 10 μg/mL INS alone, 100 μg/mL TRL alone, or 100 μg/mL TRL + 10 μg/mL INS for 8 d after 48h confluence and then stained by Oil-Red-O. Images were obtained under a microscope (×200 magnification). (B) Quantification of relative lipid accumulation was measured for absorbance at 520 nm. (C) SA-β-Gal was performed to detect senescent cells. Images were obtained under a microscope (×200 magnification). (D) SA-β-Gal positive cells were counted manually by scanning a total of 200 cells in each sample. (E) SA-β-Gal positive cells were found in both undifferentiated (only blue SA-β-Gal in the cytoplasm, marked by a hollow arrow) and differentiating (both blue SA-β-Gal and red lipid droplets in the cytoplasm, marked by a solid arrow) AMSCs. Images were obtained under a microscope (×400 magnification). Data are expressed as mean ± SD (n ≥ 3). *P < 0.05, **P < 0.01 when compared with the PBS group.