Figure 4. MiR-106a directly inhibits the expression of LATS1 and LATS2. (A, C) Schematic of miR-106a and target gene prediction based on the miRBase website. (B, D) Relative luciferase activities were analyzed in 293T cells transfected with the wild-type or mutant luciferase reporter vector for the LATS1 3'UTR (B) or the LATS2 3'UTR (D) and co-transfected with mimics-106a, mimics-NC, ASO-106a or ASO-NC. (E, G) LATS1 and LATS2 mRNA levels were analyzed via qRT-PCR in HUVECs transfected with ASO-106a or ASO-NC (E) and HCMECs transfected with mimics-106a or mimics-NC (G). (F, H) LATS1 and LATS2 protein levels were assessed using Western blotting in HUVECs transfected with ASO-106a or ASO-NC (F) and HCMECs transfected with mimics-106a or mimics-NC (H).