Research Paper Volume 13, Issue 2 pp 2668—2680

Notch-1 promotes the malignant progression of osteosarcoma through the activation of cell division cycle 20

Cdc20 overexpression abolished shRNA-mediated Notch-1 suppression. (A) The wound healing assay showed that Cdc20 upregulation abolished Notch-1 downregulation-mediated suppression of viability. (B) Quantification of the migration analysis results. *P # P C) Western blot results showing Notch-1, Cdc20, Bim and p21 expression.

Figure 6. Cdc20 overexpression abolished shRNA-mediated Notch-1 suppression. (A) The wound healing assay showed that Cdc20 upregulation abolished Notch-1 downregulation-mediated suppression of viability. (B) Quantification of the migration analysis results. *P < 0.05 vs control shRNA, # P < 0.05 vs Notch-1 shRNA or Cdc20 cDNA. (C) Western blot results showing Notch-1, Cdc20, Bim and p21 expression.