Research Paper Volume 13, Issue 1 pp 104—133

Dietary and environmental factors have opposite AhR-dependent effects on C. elegans healthspan

Loss of ahr-1 differentially regulates resistance to stressors. (A) Survival in response to heat shock. Curves show the pooled data of 60 worms in 3 independent replicates. Statistical test: Log-Rank test, * significance vs. wild-type. (B) Fertility after heat stress. Shown are the number (left panel) and viability (right panel) of eggs laid from gravid adults treated with heat shock for the indicated time. Mean + SEM of pooled data from 18 worms/condition in 3 independent experiments are shown. (C, D) Development and fertility in response to the indicated concentration of glucose. Means (+SEM) of 3 independent replicates are shown. N = number of individuals in panel (C), 9 individuals were used in panel (D). (E, F) Development and fertility in response to the indicated concentration of iron chelator (BP). Means (+SEM) of 3 and 4 independent replicates are shown. N = number of individuals in panel (E), 9 individuals were used in panel (F). (G, H) Development and fertility in response to indicated doses of UVB. Fertility was assessed at a dose of 600 J/m². Means (+SEM) of 3 independent replicates are shown. N = number of individuals in panel (G), 9 individuals were used in panel (H). (B–H) Statistical test: 2-way ANOVA with Tukey’s multiple comparisons test, * significance vs. wild-type, # significance vs. control (untreated), p-value

Figure 1. Loss of ahr-1 differentially regulates resistance to stressors. (A) Survival in response to heat shock. Curves show the pooled data of 60 worms in 3 independent replicates. Statistical test: Log-Rank test, * significance vs. wild-type. (B) Fertility after heat stress. Shown are the number (left panel) and viability (right panel) of eggs laid from gravid adults treated with heat shock for the indicated time. Mean + SEM of pooled data from 18 worms/condition in 3 independent experiments are shown. (C, D) Development and fertility in response to the indicated concentration of glucose. Means (+SEM) of 3 independent replicates are shown. N = number of individuals in panel (C), 9 individuals were used in panel (D). (E, F) Development and fertility in response to the indicated concentration of iron chelator (BP). Means (+SEM) of 3 and 4 independent replicates are shown. N = number of individuals in panel (E), 9 individuals were used in panel (F). (G, H) Development and fertility in response to indicated doses of UVB. Fertility was assessed at a dose of 600 J/m². Means (+SEM) of 3 independent replicates are shown. N = number of individuals in panel (G), 9 individuals were used in panel (H). (BH) Statistical test: 2-way ANOVA with Tukey’s multiple comparisons test, * significance vs. wild-type, # significance vs. control (untreated), p-value < 0.05.