Research Paper Volume 13, Issue 1 pp 104—133

Dietary and environmental factors have opposite AhR-dependent effects on C. elegans healthspan

At 25° C, ahr-1(ju145) is short-lived and loses its protection against the toxicity of polyQ40 aggregates. (A, B) Kaplan Meier curves of wild-type and ahr-1(ju145) at 25° C. Pooled data of 180 worms/condition in 3 independent replicates are shown. * p-value vs. wt, # p-value vs. HT115, statistical test: Log-rank test. (C, D) Kaplan Meier curves of polyQ;wt and polyQ;ahr-1. Worms were grown at 25° C from day 3 (indicated by arrowhead). Pooled data of 150 worms/condition in 3 independent replicates are shown. * p-value vs. wt, # p-value vs. HT115, statistical test: Log-rank test. (E) Representative fluorescence images of 10-days old polyQ;wt and polyQ;ahr-1 grown at 25° C from day 3. (F) Quantification of aggregates in 10-days old polyQ;wt and polyQ;ahr-1 grown at 25° C from day 3. Mean + 95 % CI of pooled data from 21 (wt HT115), 19 (wt OP50), 20 (ahr-1 HT115) and 19 (ahr-1 OP50) worms/condition in 2 independent replicates are shown. * p-value vs. wt, statistical test: One-way ANOVA with Tukey’s multiple comparisons test.

Figure 4. At 25° C, ahr-1(ju145) is short-lived and loses its protection against the toxicity of polyQ40 aggregates. (A, B) Kaplan Meier curves of wild-type and ahr-1(ju145) at 25° C. Pooled data of 180 worms/condition in 3 independent replicates are shown. * p-value < 0.05 vs. wt, # p-value < 0.05 vs. HT115, statistical test: Log-rank test. (C, D) Kaplan Meier curves of polyQ;wt and polyQ;ahr-1. Worms were grown at 25° C from day 3 (indicated by arrowhead). Pooled data of 150 worms/condition in 3 independent replicates are shown. * p-value < 0.05 vs. wt, # p-value < 0.05 vs. HT115, statistical test: Log-rank test. (E) Representative fluorescence images of 10-days old polyQ;wt and polyQ;ahr-1 grown at 25° C from day 3. (F) Quantification of aggregates in 10-days old polyQ;wt and polyQ;ahr-1 grown at 25° C from day 3. Mean + 95 % CI of pooled data from 21 (wt HT115), 19 (wt OP50), 20 (ahr-1 HT115) and 19 (ahr-1 OP50) worms/condition in 2 independent replicates are shown. * p-value < 0.05 vs. wt, statistical test: One-way ANOVA with Tukey’s multiple comparisons test.