Research Paper Advance Articles

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium

High-salt promotes adhesion molecules expression and inflammatory cells infiltration in ECs via PAI-1. (A–D) mRNA expression of adhesion molecules (E-selectin, VCAM-1, ICAM-1, and MCP-1) in TA and AA regions of ApoE-/- mice in normal and high salt groups after 4 weeks feeding. (E–H) mRNA expression of adhesion molecules (E-selectin, VCAM-1, ICAM-1, and MCP-1) in HUVECs that were treated with Ctr siRNA or PAI-1 siRNA under high-salt condition for two days. (I) Representative images of adherent monocytes to HUVECs that were treated with Ctr siRNA or PAI-1 siRNA under high-salt condition. (J) Representative images of infiltrated monocytes into HUVECs that were treated with Ctr siRNA or PAI-1 siRNA under high-salt condition. All data were presented as mean ± SEM, N≥3. *p

Figure 3. High-salt promotes adhesion molecules expression and inflammatory cells infiltration in ECs via PAI-1. (AD) mRNA expression of adhesion molecules (E-selectin, VCAM-1, ICAM-1, and MCP-1) in TA and AA regions of ApoE-/- mice in normal and high salt groups after 4 weeks feeding. (EH) mRNA expression of adhesion molecules (E-selectin, VCAM-1, ICAM-1, and MCP-1) in HUVECs that were treated with Ctr siRNA or PAI-1 siRNA under high-salt condition for two days. (I) Representative images of adherent monocytes to HUVECs that were treated with Ctr siRNA or PAI-1 siRNA under high-salt condition. (J) Representative images of infiltrated monocytes into HUVECs that were treated with Ctr siRNA or PAI-1 siRNA under high-salt condition. All data were presented as mean ± SEM, N≥3. *p < 0.05 versus control group.