Research Paper Volume 13, Issue 3 pp 3866—3885

Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer

The effect of miR-581 on cell proliferation, migration, and invasion. (A, B) Colony formation of (A) FHC cells transfected with miR-581 mimics and (B) Cell proliferation of LoVo cells transfected with miR-581 antagomir tested by colony formation assay. (C) The percentage of Ki-67-positive FHC cells increases significantly after transfection with miR-581 mimics. (D) The percentage of Ki-67-positive LoVo cells is significantly lower after transfection with miR-581 antagomir. (E, F) Wound closure rate of (E). FHC cells transfected with miR-581 mimics and (F) LoVo cells transfected with miR-581 antagomir subjected to the in vitro scratch assay. (G, H) Migratory and invasive abilities of (G) FHC cells transfected with miR-581 mimics and (H) LoVo cells transfected with miR-581 antagomir evaluated by Transwell migration and invasion assays. Comparison between two groups were assessed by student’s t-test. Bars were represented as S.D. *PPP

Figure 3. The effect of miR-581 on cell proliferation, migration, and invasion. (A, B) Colony formation of (A) FHC cells transfected with miR-581 mimics and (B) Cell proliferation of LoVo cells transfected with miR-581 antagomir tested by colony formation assay. (C) The percentage of Ki-67-positive FHC cells increases significantly after transfection with miR-581 mimics. (D) The percentage of Ki-67-positive LoVo cells is significantly lower after transfection with miR-581 antagomir. (E, F) Wound closure rate of (E). FHC cells transfected with miR-581 mimics and (F) LoVo cells transfected with miR-581 antagomir subjected to the in vitro scratch assay. (G, H) Migratory and invasive abilities of (G) FHC cells transfected with miR-581 mimics and (H) LoVo cells transfected with miR-581 antagomir evaluated by Transwell migration and invasion assays. Comparison between two groups were assessed by student’s t-test. Bars were represented as S.D. *P<0.05; **P<0.01; ***P<0.001.