Research Paper Volume 13, Issue 3 pp 3866—3885

Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer

LINC00485 regulates cell proliferation, migration, and invasion by sponging miR-581. (A, B) Cell viability of LINC00485 knockdown FHC cells with or without miR-581 antagomir treatment for 24 h and (B) LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was evaluated by the CCK-8 assay. (C) The percentage of Ki-67-positive cells in (C) LINC00485 knockdown FHC cells with or without miR-581 antagomir treatment for 24 h was tested by flow cytometry. (D) The percentage of Ki-67-positive cells in LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was evaluated by flow cytometry. (E, F) Colony forming capability of (E) LINC00485 silenced FHC cells with or without miR-581 antagomir treatment for 24 h and (F) LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was measured by the colony formation assay. (G, H) The migratory and invasive abilities of (G) LINC00485-silenced FHC cells with or without miR-581 antagomir treatment for 24 h and (H) LINC00485-overexpressed LoVo cells with or without miR-581 mimics for 24 h were detected by Transwell assays. Data was analyzed using the one-way ANOVA with LSD test. Bars were represented as S.D. *PLINC00485; OE, overexpression.

Figure 4. LINC00485 regulates cell proliferation, migration, and invasion by sponging miR-581. (A, B) Cell viability of LINC00485 knockdown FHC cells with or without miR-581 antagomir treatment for 24 h and (B) LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was evaluated by the CCK-8 assay. (C) The percentage of Ki-67-positive cells in (C) LINC00485 knockdown FHC cells with or without miR-581 antagomir treatment for 24 h was tested by flow cytometry. (D) The percentage of Ki-67-positive cells in LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was evaluated by flow cytometry. (E, F) Colony forming capability of (E) LINC00485 silenced FHC cells with or without miR-581 antagomir treatment for 24 h and (F) LINC00485-overexpressed LoVo cells with or without miR-581 mimics treatment for 24 h was measured by the colony formation assay. (G, H) The migratory and invasive abilities of (G) LINC00485-silenced FHC cells with or without miR-581 antagomir treatment for 24 h and (H) LINC00485-overexpressed LoVo cells with or without miR-581 mimics for 24 h were detected by Transwell assays. Data was analyzed using the one-way ANOVA with LSD test. Bars were represented as S.D. *P<0.05. sh, short hairpin RNA targeting LINC00485; OE, overexpression.