Research Paper Volume 13, Issue 3 pp 3866—3885

Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer

LINC00485/miR-581/EDEM1 axis regulates epithelial to mesenchymal transition in CRC cells. (A) The expression of EDEM1 in LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1. (B) Cell viability of LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1 measured by the CCK-8 assay. (C–E) The (C, E) migratory and (D) invasive capabilities of LoVo cells transfected with miR-581 antagomir or co-transfected with miR-581 antagomir and siEDEM1 were assessed by Transwell migration and invasion assays and the in vitro scratch assay. (F) The mRNA levels of cytokeratin, E-cadherin, N-cadherin, and vimentin in LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1 were measured by RT-qPCR. (G–K) The protein levels of (C) EDEM1, (D) Cytokeratin, (E) E-cadherin, (F) N-cadherin and (G) Vimentin in LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1 were quantified by western blotting assay. Data were analyzed using one-way ANOVA with LSD test. Bars were represented as S.D. *PP

Figure 7. LINC00485/miR-581/EDEM1 axis regulates epithelial to mesenchymal transition in CRC cells. (A) The expression of EDEM1 in LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1. (B) Cell viability of LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1 measured by the CCK-8 assay. (CE) The (C, E) migratory and (D) invasive capabilities of LoVo cells transfected with miR-581 antagomir or co-transfected with miR-581 antagomir and siEDEM1 were assessed by Transwell migration and invasion assays and the in vitro scratch assay. (F) The mRNA levels of cytokeratin, E-cadherin, N-cadherin, and vimentin in LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1 were measured by RT-qPCR. (GK) The protein levels of (C) EDEM1, (D) Cytokeratin, (E) E-cadherin, (F) N-cadherin and (G) Vimentin in LoVo cells transfected with miR-581 antagomir or in combination with siEDEM1 were quantified by western blotting assay. Data were analyzed using one-way ANOVA with LSD test. Bars were represented as S.D. *P<0.05; **P<0.01; NS, not significant; OE, overexpression; si, small interfering RNA targeting EDEM1; EDEM1, ER-degradation-enhancing alpha-mannosidase-like protein-1.