Research Paper Volume 13, Issue 3 pp 3886—3897

Praja2 suppresses the growth of gastric cancer by ubiquitylation of KSR1 and inhibiting MEK-ERK signal pathways

Silencing of KSR1 inhibited the progression of GC cells. (A) The silencing efficiency of si-KSR1 in MKN-45 cells. (B) Western blot was to measure the protein level of KSR1 in MKN-45 cells. (C) CCK8 was to determine the viability of MKN-45 cells. (D) Edu assay was to detect proliferation of MKN-45 cells. Scale bar, 40 μm. (E) Wound healing assay was to evaluate migration of MKN-45 cells. Scale bar, 60 μm. (F) Transwell assay was to examine invasion of MKN-45 cells. Scale bar, 60 μm. (G) Flow cytometry assay was to determine apoptosis of MKN-45 cells. Data are mean ± SD; *P

Figure 2. Silencing of KSR1 inhibited the progression of GC cells. (A) The silencing efficiency of si-KSR1 in MKN-45 cells. (B) Western blot was to measure the protein level of KSR1 in MKN-45 cells. (C) CCK8 was to determine the viability of MKN-45 cells. (D) Edu assay was to detect proliferation of MKN-45 cells. Scale bar, 40 μm. (E) Wound healing assay was to evaluate migration of MKN-45 cells. Scale bar, 60 μm. (F) Transwell assay was to examine invasion of MKN-45 cells. Scale bar, 60 μm. (G) Flow cytometry assay was to determine apoptosis of MKN-45 cells. Data are mean ± SD; *P < 0.05.