Research Paper Volume 13, Issue 3 pp 3909—3925

Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression

Up-regulation of PYCR1 in bladder cancer. (A) Venn diagram showing commonly upregulated BLCA signatures in both GEPIA and Cancer RNA-seq Nexus databases. (B) Overall survival in BLCA patients with the higher PYCR1 level was shorter as demonstrated by Kaplan-Meier analysis. (C, D) Determining PYCR1 mRNA (C) and protein (D) in patient-derived bladder tumor (T) versus normal tissues (N). * p t-test. (E, F) PYCR1 protein (E) and mRNA (F) expression in the representative BLCA cells lines. (G) PYCR1 mRNA level upon USP18 depletion by qPCR. *** p t-test.

Figure 5. Up-regulation of PYCR1 in bladder cancer. (A) Venn diagram showing commonly upregulated BLCA signatures in both GEPIA and Cancer RNA-seq Nexus databases. (B) Overall survival in BLCA patients with the higher PYCR1 level was shorter as demonstrated by Kaplan-Meier analysis. (C, D) Determining PYCR1 mRNA (C) and protein (D) in patient-derived bladder tumor (T) versus normal tissues (N). * p < 0.05, *** p < 0.001, student’s t-test. (E, F) PYCR1 protein (E) and mRNA (F) expression in the representative BLCA cells lines. (G) PYCR1 mRNA level upon USP18 depletion by qPCR. *** p < 0.001, student’s t-test.