Research Paper Volume 13, Issue 3 pp 3945—3956

MicroRNA-205-5p targets the HOXD9-Snail1 axis to inhibit triple negative breast cancer cell proliferation and chemoresistance

HOXD9 transcription activates Snail1 expression. (A) Verifying the correlation between HOXD9 and Snail1 mRNA in 100 TNBC specimens. (B) The correlation between HOXD9 and Snail1 mRNA expression was calculated in GEPIA database. (C) HOXD9 knockdown treatment could effectively inhibit Snail1 protein expression in BT549 cells. (D) Western blot analysis of the Snail1 protein expression in HOXD9 knockdown treated BT549 cells. (E) qRT-PCR analysis of the Snail1 mRNA expression in HOXD9 knockdown treated BT549 cells. (F) ChIP experiments was used to verify the binding site between HOXD9 and the promoter region of Snail1 gene. (G) Dual luciferase experiment was employed to verify the binding site between HOXD9 and the wild-type promoter region of Snail1. (H) Western blot analysis of the Snail1 protein expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. (I) qRT-PCR analysis of the Snail1 mRNA expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. **P

Figure 6. HOXD9 transcription activates Snail1 expression. (A) Verifying the correlation between HOXD9 and Snail1 mRNA in 100 TNBC specimens. (B) The correlation between HOXD9 and Snail1 mRNA expression was calculated in GEPIA database. (C) HOXD9 knockdown treatment could effectively inhibit Snail1 protein expression in BT549 cells. (D) Western blot analysis of the Snail1 protein expression in HOXD9 knockdown treated BT549 cells. (E) qRT-PCR analysis of the Snail1 mRNA expression in HOXD9 knockdown treated BT549 cells. (F) ChIP experiments was used to verify the binding site between HOXD9 and the promoter region of Snail1 gene. (G) Dual luciferase experiment was employed to verify the binding site between HOXD9 and the wild-type promoter region of Snail1. (H) Western blot analysis of the Snail1 protein expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. (I) qRT-PCR analysis of the Snail1 mRNA expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. **P<0.01,***P<0.001.