Figure 4. PRPS2 mediates PSCs stemness by PRPS2-regulated purine metabolism. (A, B) WB (A) and qRT-PCR (B) analysis of effects of PRPS2 KO or re-expression on PRPS1 expression in PSCs. WT, sgPRPS2 resistant WT PRPS2. (C) Effect of 6-MP treatment on PRPS2 KO or re-expression PSCs’ viabilities. (D) Heatmap showing metabolomics analysis in PSCs from (A). (E, F) qRT-PCR analysis of effects of PRPS2 KO or re-expression on the expression of pluripotency genes or triploblastic genes in PSCs (E) and EBs (embryoid bodies) (F). (G) qRT-PCR analysis of effects of PRPS2 on the expression of pluripotency genes and triploblastic genes in response to HX treatment. In (B, E, F and G), data are expressed as the mean ± SD. *P <0.05, **P <0.01, ***P <0.001, by two-tailed t-test. Data are representative of three independent experiments with similar results.