Research Paper Volume 13, Issue 3 pp 4079—4095

Microglia exosomal miRNA-137 attenuates ischemic brain injury through targeting Notch1

M2-phenotype microglia-derived exosomes (BV2-Exo) attenuated neuronal apoptosis induced oxygen-glucose deprivation (OGD) through miRNA-137. (A) Protein expressions of caspase-3 and cleaved caspase-3 in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC, as detected by Western blotting assay. (B) Lactate dehydrogenase (LDH) assay for detecting LDH activity in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC. (C) LDH assay for detecting LDH activity in neurons treated with 1) control, 2) BV2-Exo, 3) BV2-Exo plus BV2-Exo+miRNA-137-IN, and 4) BV2-Exo plus BV2-Exo+miRNA-137-NC. (D) TUNEL assay for detecting apoptosis in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC. Data are presented as mean±SD. *, p

Figure 5. M2-phenotype microglia-derived exosomes (BV2-Exo) attenuated neuronal apoptosis induced oxygen-glucose deprivation (OGD) through miRNA-137. (A) Protein expressions of caspase-3 and cleaved caspase-3 in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC, as detected by Western blotting assay. (B) Lactate dehydrogenase (LDH) assay for detecting LDH activity in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC. (C) LDH assay for detecting LDH activity in neurons treated with 1) control, 2) BV2-Exo, 3) BV2-Exo plus BV2-Exo+miRNA-137-IN, and 4) BV2-Exo plus BV2-Exo+miRNA-137-NC. (D) TUNEL assay for detecting apoptosis in neurons treated with 1) control, 2) OGD, 3) OGD plus BV2-Exo, 4) OGD plus BV2-Exo+miRNA-137-IN, and 5) OGD plus BV2-Exo+miRNA-137-NC. Data are presented as mean±SD. *, p<0.05. At least three replicates were available for statistical analysis in each treatment.