Research Paper Volume 13, Issue 1 pp 1458—1472

CHIP protects against MPP+/MPTP-induced damage by regulating Drp1 in two models of Parkinson’s disease

CHIP prevented the upregulation of Drp1 induced by MPP+. (A, B) Immunoblot of Drp1 in different groups of MPP+-induced PD cell models. MPP+ caused the increase of Drp1 in SH-SY5Y cells. After transfecting with CHIP plasmid, the rising trend of Drp1 was inhibited. (C, D) Drp1 levels in MPP+-induced cells with or without Mdivi-1 treated. Mdivi-1 could prevent the increase of Drp1 caused by MPP+. (E–G) Under the condition of CHIP knockdown, LDH and ATP levels in MPP+-induced cells with or without Mdivi-1 treated were detected. *P

Figure 3. CHIP prevented the upregulation of Drp1 induced by MPP+. (A, B) Immunoblot of Drp1 in different groups of MPP+-induced PD cell models. MPP+ caused the increase of Drp1 in SH-SY5Y cells. After transfecting with CHIP plasmid, the rising trend of Drp1 was inhibited. (C, D) Drp1 levels in MPP+-induced cells with or without Mdivi-1 treated. Mdivi-1 could prevent the increase of Drp1 caused by MPP+. (EG) Under the condition of CHIP knockdown, LDH and ATP levels in MPP+-induced cells with or without Mdivi-1 treated were detected. *P<0.05.