Research Paper Volume 13, Issue 3 pp 4335—4356

CUEDC2 ablation enhances the efficacy of mesenchymal stem cells in ameliorating cerebral ischemia/reperfusion insult

Cerebral I/R induced the downregulation of CUEDC2 expression in brain tissues. (A–C) Protein and mRNA expression of CUEDC2 in neurons as detected by western blotting and PCR assays. (D–F) Protein and mRNA expression of CUEDC2 in brain tissues as assayed by western blotting and PCR. (G, H) Protein expression of CUEDC2 in brain tissues as assayed by immunohistochemical staining. Scale bar = 50 μm. CTR, control; CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (0,4,8,12, and 24 hours); MCAO, middle cerebral artery occlusion. All data are presented as the mean value ± SD. *p

Figure 1. Cerebral I/R induced the downregulation of CUEDC2 expression in brain tissues. (AC) Protein and mRNA expression of CUEDC2 in neurons as detected by western blotting and PCR assays. (DF) Protein and mRNA expression of CUEDC2 in brain tissues as assayed by western blotting and PCR. (G, H) Protein expression of CUEDC2 in brain tissues as assayed by immunohistochemical staining. Scale bar = 50 μm. CTR, control; CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (0,4,8,12, and 24 hours); MCAO, middle cerebral artery occlusion. All data are presented as the mean value ± SD. *p<0.05, **p<0.01; compared to the control group, sham group.