Research Paper Volume 13, Issue 3 pp 4335—4356

CUEDC2 ablation enhances the efficacy of mesenchymal stem cells in ameliorating cerebral ischemia/reperfusion insult

CUEDC2 knockdown in MSCs enhances the inhibitory effect on OGD/R-induced apoptotic cell death in co-cultured neurons. (A, B) Apoptotic cell death in co-cultured neurons after treatment with different MSCs as detected by flow cytometry with Annexin V/PI staining. (C) Apoptotic cell death in co-cultured neurons after treatment with different MSCs as analyzed by LDH-leakage assay. (D) Viability of co-cultured neurons after treatment with different MSCs as evaluated by MTT assay. (E, F) Caspase-3 protein expression in co-cultured neurons after treatment with different MSCs as assayed by western blotting. CTR: control; MSCs: mesenchymal stem cells; CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (24 hours); MSCs- siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs. All data are presented as the mean value ± SD. *p

Figure 4. CUEDC2 knockdown in MSCs enhances the inhibitory effect on OGD/R-induced apoptotic cell death in co-cultured neurons. (A, B) Apoptotic cell death in co-cultured neurons after treatment with different MSCs as detected by flow cytometry with Annexin V/PI staining. (C) Apoptotic cell death in co-cultured neurons after treatment with different MSCs as analyzed by LDH-leakage assay. (D) Viability of co-cultured neurons after treatment with different MSCs as evaluated by MTT assay. (E, F) Caspase-3 protein expression in co-cultured neurons after treatment with different MSCs as assayed by western blotting. CTR: control; MSCs: mesenchymal stem cells; CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (24 hours); MSCs- siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs. All data are presented as the mean value ± SD. *p<0.05, **p<0.01; compared to the control group, MSCs-vector group.