Research Paper Volume 13, Issue 3 pp 4335—4356

CUEDC2 ablation enhances the efficacy of mesenchymal stem cells in ameliorating cerebral ischemia/reperfusion insult

CUEDC2 degradation in MSCs improves protection against OGD/R-induced apoptotic cell death in co-cultured neurons by suppressing oxidative toxicity. (A) ROS production in co-cultured neurons after treatment with different MSCs as detected by DCFH-DA assay. (B) MDA production in co-cultured neurons after treatment with different MSCs as evaluated by lipid peroxidation MDA assay. (C) SOD production in co-cultured neurons after treatment with different MSCs as determined by WST-8 assay. (D) T-AOC level in co-cultured neurons after treatment with different MSCs as detected by ABTS assay. (E) ROS production in co-cultured neurons after treatment with different MSCs exposed to H2O2 as analyzed by DCFH-DA assay. (F) MDA production in co-cultured neurons after treatment with different MSCs exposed to H2O2 as evaluated by lipid peroxidation MDA assay. (G) SOD production in co-cultured neurons after treatment with different MSCs exposed to H2O2 as determined by WST-8 assay. (H) T-AOC level in co-cultured neurons after treatment with different MSCs exposed to H2O2 as detected by ABTS assay. (I) Co-cultured neuron viability after treatment with different MSCs subjected to H2O2 as evaluated by MTT analysis. (J) Apoptosis in co-cultured neurons after treatment with different MSCs subjected to H2O2 as evaluated by LDH leakage assay. CTR: control; MSCs: mesenchymal stem cells; CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (24 hours). MSCs- siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs. All data are presented as the mean value ± SD. *P2O2 treatment groups.

Figure 5. CUEDC2 degradation in MSCs improves protection against OGD/R-induced apoptotic cell death in co-cultured neurons by suppressing oxidative toxicity. (A) ROS production in co-cultured neurons after treatment with different MSCs as detected by DCFH-DA assay. (B) MDA production in co-cultured neurons after treatment with different MSCs as evaluated by lipid peroxidation MDA assay. (C) SOD production in co-cultured neurons after treatment with different MSCs as determined by WST-8 assay. (D) T-AOC level in co-cultured neurons after treatment with different MSCs as detected by ABTS assay. (E) ROS production in co-cultured neurons after treatment with different MSCs exposed to H2O2 as analyzed by DCFH-DA assay. (F) MDA production in co-cultured neurons after treatment with different MSCs exposed to H2O2 as evaluated by lipid peroxidation MDA assay. (G) SOD production in co-cultured neurons after treatment with different MSCs exposed to H2O2 as determined by WST-8 assay. (H) T-AOC level in co-cultured neurons after treatment with different MSCs exposed to H2O2 as detected by ABTS assay. (I) Co-cultured neuron viability after treatment with different MSCs subjected to H2O2 as evaluated by MTT analysis. (J) Apoptosis in co-cultured neurons after treatment with different MSCs subjected to H2O2 as evaluated by LDH leakage assay. CTR: control; MSCs: mesenchymal stem cells; CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (24 hours). MSCs- siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs. All data are presented as the mean value ± SD. *P<0.05, **P<0.01; compared with the control, MSCs-vector, and H2O2 treatment groups.