Research Paper Volume 13, Issue 3 pp 4335—4356

CUEDC2 ablation enhances the efficacy of mesenchymal stem cells in ameliorating cerebral ischemia/reperfusion insult

CUEDC2 ablation promotes the efficacy of MSCs in I/R-induced brain damages. (A) Illustration of MCAO-induced model of cerebral I/R in rats and different MSCs treatment. SD rats were exposed to MCAO for 90 minutes and subsequent reperfusion. After 24-hours of reperfusion, the CUEDC2-modified MSCs and the MSCs vector were infused on MCAO model rats. (B) Evaluation of neurologic severity scores. (C, D) Assessment of infarct size with TTC staining in whole-brain tissues. (E) Determination of brain water content. (F) H&E staining in brain tissues. Scale bar = 100 μm. MSCs: mesenchymal stem cells; MSCs- siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs; MCAO, middle cerebral artery occlusion. All data are presented as the mean value ± SD. *p

Figure 6. CUEDC2 ablation promotes the efficacy of MSCs in I/R-induced brain damages. (A) Illustration of MCAO-induced model of cerebral I/R in rats and different MSCs treatment. SD rats were exposed to MCAO for 90 minutes and subsequent reperfusion. After 24-hours of reperfusion, the CUEDC2-modified MSCs and the MSCs vector were infused on MCAO model rats. (B) Evaluation of neurologic severity scores. (C, D) Assessment of infarct size with TTC staining in whole-brain tissues. (E) Determination of brain water content. (F) H&E staining in brain tissues. Scale bar = 100 μm. MSCs: mesenchymal stem cells; MSCs- siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs; MCAO, middle cerebral artery occlusion. All data are presented as the mean value ± SD. *p<0.05, **p<0.01; compared to sham group, the vector of MSC group.