Research Paper Volume 13, Issue 3 pp 4335—4356

CUEDC2 ablation enhances the efficacy of mesenchymal stem cells in ameliorating cerebral ischemia/reperfusion insult

CUEDC2 silencing in MSCs upregulates the antioxidant effect on co-cultured neurons by increasing GPX1 expression. (A, B) GPX1 protein expression in co-cultured neurons under non-OGD/R condition as detected by western blotting. (C, D) GPX1 protein expression in co-cultured neurons under OGD/R condition as detected by western blotting. (E) ROS production in co-cultured neurons after treatment with various MSCs as analyzed by DCFH-DA assay. (F) MDA production in co-cultured neurons after treatment with various MSCs as evaluated by lipid peroxidation MDA assay. (G) SOD production in co-cultured neurons after treatment with various MSCs as determined by WST-8 assay. (H) T-AOC level in co-cultured neurons after treatment with various MSCs as detected by ABTS assay. (I) Viability of co-cultured neurons after treatment with various MSCs as evaluated by MTT analysis. (J) Apoptosis in co-cultured neurons after treatment with various MSCs as evaluated by LDH leakage assay. (K, L) Apoptosis in co-cultured neurons after treatment with various MSCs as detected by Hoechst staining. CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (12- or 24-hours). MSCs-siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs; siRNA-GPX1: small interfering RNA knockdown of GPX1 in MSC; All data are presented as the mean value ± SD. *p

Figure 7. CUEDC2 silencing in MSCs upregulates the antioxidant effect on co-cultured neurons by increasing GPX1 expression. (A, B) GPX1 protein expression in co-cultured neurons under non-OGD/R condition as detected by western blotting. (C, D) GPX1 protein expression in co-cultured neurons under OGD/R condition as detected by western blotting. (E) ROS production in co-cultured neurons after treatment with various MSCs as analyzed by DCFH-DA assay. (F) MDA production in co-cultured neurons after treatment with various MSCs as evaluated by lipid peroxidation MDA assay. (G) SOD production in co-cultured neurons after treatment with various MSCs as determined by WST-8 assay. (H) T-AOC level in co-cultured neurons after treatment with various MSCs as detected by ABTS assay. (I) Viability of co-cultured neurons after treatment with various MSCs as evaluated by MTT analysis. (J) Apoptosis in co-cultured neurons after treatment with various MSCs as evaluated by LDH leakage assay. (K, L) Apoptosis in co-cultured neurons after treatment with various MSCs as detected by Hoechst staining. CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (12- or 24-hours). MSCs-siRNA-CUEDC2: small interfering RNA silencing CUEDC2 in MSCs; MSCs-vector: the vector of MSCs; siRNA-GPX1: small interfering RNA knockdown of GPX1 in MSC; All data are presented as the mean value ± SD. *p<0.05, **p<0.01; comparison to MSC-vector group.