Research Paper Volume 12, Issue 24 pp 26199—26220

Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1

Figure 2. The downregulation of RNF2 inhibited cell proliferation and increased apoptosis in CRC cells. (A) Western blot analysis showing the knockdown efficiency of RNF2 in HCT116 cells that had been infected with shRNA-expressing lentiviruses for 48 hours. (B) MTT assay showing the proliferation of RNF2-knockdown and control HCT116 cells. (C) Apoptosis analysis of RNF2-knockdown and control HCT116 cells. (D) MTT assay showing the proliferation of RNF2-knockdown and control WiDr cells. (E) Apoptosis analysis of RNF2-knockdown and control WiDr cells. (F) Western blot analysis showing the knockdown efficiency of RNF2 and the cleavage of PARP in RNF2-knockdown and control WiDr cells. (G) qRT-PCR analysis showing the mRNA levels of IL-6 and IL-8 in RNF2-knockdown and control HCT116 cells. (H) ELISA assay showing the soluble IL-6 and IL-8 levels in culture media. Three independent experiments were performed and analyzed for B-E. Data represent the mean ± standard deviation. *p<0.05 versus shCon group.