Research Paper Volume 12, Issue 24 pp 26199—26220

Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1

Figure 4. Knocking down EGR1 partially reversed the inhibition of cell proliferation and the increase in apoptosis in RNF2-knockdown cells. (A) Western blot showing the knockdown efficiency of RNF2 and EGR1 and the cleavage of PARP in RNF2/EGR1 double-knockdown, RNF2-knockdown and control HCT116 cells. (B) MTT assay showing the proliferation of RNF2/EGR1 double-knockdown, RNF2-knockdown and control HCT116 cells. (C) Apoptosis analysis of RNF2/EGR1 double-knockdown, RNF2-knockdown and control HCT116 cells. Three independent experiments were performed and analyzed for B and C. Data represent the mean ± standard deviation. *p<0.05 versus shCon, Δp<0.05 versus shRNF2.