Research Paper Volume 12, Issue 24 pp 26199—26220

Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1

Figure 5. The downregulation of RNF2 reduced both RNF2 enrichment and H2A mono-ubiquitination at the EGR1 promoter. (A) The positions of the three PCR primer sets used in the ChIP assay. (B) Quantitative PCR analysis after a ChIP assay using antibodies against RNF2 and H2A K119Ub. Three independent experiments were performed and analyzed. Data represent the mean ± standard deviation. *p<0.05, **p<0.01 and ***p<0.001 versus IgG. (C) Quantitative PCR analysis using primer set 2 after a ChIP assay to show the enrichment of RNF2 and the mono-ubiquitination of H2A at the EGR1 promotor in RNF2-knockdown and control HCT116 cells. Three independent experiments were performed and analyzed. Data represent the mean ± standard deviation. *p<0.05, **p<0.01 and ***p<0.001 versus shCon.