Research Paper Volume 13, Issue 6 pp 8055—8067

Silencing SIX1 by miR-7160 inhibits non-small cell lung cancer cell growth

miR-7160 overexpression inhibits NSCLC cell growth, proliferation, migration and invasion. The primary NSCLC cells (pCan-1/pCan-2/pCan-3) or A549 cells, bearing the pre-miR-7160-expression lentiviral construct (“lv-pre-miR-7160”) or non-sense miRNA control lentiviral construct (“lv-miRC”), were cultured for applied time periods; Cellular functions, including viability (A, G), growth (B), proliferation (C, D, H), migration (E, I) and invasion (F) were tested by the assays mentioned in the text, with results quantified. Data were presented as mean ± SD (n=5), and results normalized. *PD–F).

Figure 2. miR-7160 overexpression inhibits NSCLC cell growth, proliferation, migration and invasion. The primary NSCLC cells (pCan-1/pCan-2/pCan-3) or A549 cells, bearing the pre-miR-7160-expression lentiviral construct (“lv-pre-miR-7160”) or non-sense miRNA control lentiviral construct (“lv-miRC”), were cultured for applied time periods; Cellular functions, including viability (A, G), growth (B), proliferation (C, D, H), migration (E, I) and invasion (F) were tested by the assays mentioned in the text, with results quantified. Data were presented as mean ± SD (n=5), and results normalized. *P< 0.05 vs. “lv-miRC” cells. Experiments in this figure were repeated five times with similar results obtained. Bar= 100 μm (DF).