Research Paper Volume 13, Issue 6 pp 8055—8067

Silencing SIX1 by miR-7160 inhibits non-small cell lung cancer cell growth

miR-7160 inhibition promotes NSCLC cell progression. The pCan-1 primary NSCLC cells, bearing the pre-miR-7160 anti-sense lentiviral construct (“lv-antagomiR-7160”) or non-sense anti-sense control lentiviral construct (“lv-antagomiRC”), were established, and cells cultured for applied time periods; Expression of listed genes was tested by qPCR and Western blotting analyses (A, C, D), with SIX1 3’-UTR luciferase reporter activity tested as well (B). Cellular functions, including cell proliferation (E), migration (F) and invasion (G) were tested by the assays mentioned in the text, with results quantified. Data were presented as mean ± SD (n=5), and results normalized. *P

Figure 4. miR-7160 inhibition promotes NSCLC cell progression. The pCan-1 primary NSCLC cells, bearing the pre-miR-7160 anti-sense lentiviral construct (“lv-antagomiR-7160”) or non-sense anti-sense control lentiviral construct (“lv-antagomiRC”), were established, and cells cultured for applied time periods; Expression of listed genes was tested by qPCR and Western blotting analyses (A, C, D), with SIX1 3’-UTR luciferase reporter activity tested as well (B). Cellular functions, including cell proliferation (E), migration (F) and invasion (G) were tested by the assays mentioned in the text, with results quantified. Data were presented as mean ± SD (n=5), and results normalized. *P< 0.05 vs. “lv-antagomiRC” cells. Experiments in this figure were repeated five times with similar results obtained.