Research Paper Volume 13, Issue 3 pp 4370—4387

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation

Characterization of ADSCs. (A) Microscopic image showing spindle-shaped, third-passage ADSCs. (B) Oil red O staining for adipocytes. (C) Alizarin Red S staining for osteocytes. (D) Von Kossa staining for osteocytes. (E) Human ADSC characterization using flow cytometry. Isolated ADSCs showed high expression of CD13, CD44, CD73, CD90, and CD105 (positive) and very low expression of HLA-DR, CD34, and CD45 (negative).

Figure 1. Characterization of ADSCs. (A) Microscopic image showing spindle-shaped, third-passage ADSCs. (B) Oil red O staining for adipocytes. (C) Alizarin Red S staining for osteocytes. (D) Von Kossa staining for osteocytes. (E) Human ADSC characterization using flow cytometry. Isolated ADSCs showed high expression of CD13, CD44, CD73, CD90, and CD105 (positive) and very low expression of HLA-DR, CD34, and CD45 (negative).