Research Paper Volume 13, Issue 3 pp 4370—4387

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation

Inhibited the expression of PLOD2 by minoxidil, the neurorestorative effect of ADSCs was weakened. (A) LDH release assay result from MI-exposed cells co-cultured with ADSCs in the presence or absence of minoxidil. (B) Analysis of PLOD2, MAP2, NSE, GAP43, and GFAP transcript expression in MI-treated neurons co-cultured with ADSCs in the presence or absence of minoxidil. (C) Western blot analysis of PLOD2 and neuronal markers. Results presented as mean ± SD and evaluated with one-way ANOVA. *PP

Figure 4. Inhibited the expression of PLOD2 by minoxidil, the neurorestorative effect of ADSCs was weakened. (A) LDH release assay result from MI-exposed cells co-cultured with ADSCs in the presence or absence of minoxidil. (B) Analysis of PLOD2, MAP2, NSE, GAP43, and GFAP transcript expression in MI-treated neurons co-cultured with ADSCs in the presence or absence of minoxidil. (C) Western blot analysis of PLOD2 and neuronal markers. Results presented as mean ± SD and evaluated with one-way ANOVA. *P<0.05, compared to control (no MI) and MI/ADSC groups; #P<0.05, compared to MI/ADSC and control (DMSO/MI/ADSC) groups.