Research Paper Volume 12, Issue 24 pp 24836—24852

miR-106b suppresses pathological retinal angiogenesis

Figure 2. PERK activation provokes downregulation of miR-106b-25 cluster and MCM7 host gene in a mouse model of CNV. (A) Schematic of ER stress activation of PERK pathway. (B) Western blot of Phospho-EIF2α, total-EIF2α and β-actin in control choroids, and 3 and 7 days after burns. (C) Western blot quantification in control choroids, 3 days (n=7) and 7 days after burns (n=6). (D) Aft4 mRNA expression in control choroids, 3 days (n=6) and 7 days after burns (n=10). (E) Mcm7 mRNA expression in control choroids, 3 days (n=6) and 7 days after laser burn (n=14). (F) miR106~25 cluster member expression in choroids 3 days after laser burn (miR-106b (n=7), miR-25 (n=6) and miR-93 (n=5). (G) miR-106b expression after infection of HRMECs with LV.shGFP (negative control), and LV.shPERK (n=5). Data are expressed as mean ± S.E.M. One-way ANOVA with Bonferroni post-hoc test was performed on groups of 3 or more, and unpaired Two-tailed Student’s t-test was used for the analysis of groups of 2, *P <0.05; **P<0.001; ***P<0.0001.