Figure 4. Analysis of 219 hub genes with degree ≥10. GO biological process analysis using the BinGo plug-in of Cytoscape (A). KEGG pathway analysis using the ClueGo plug-in of Cytoscape (B). Volcano plot of the enriched pathways with P values and enrichment scores (C). Altered genes in the Wnt signaling pathway (D). Altered genes in the MAPK signaling pathway (E). Orange and cyan rectangles indicate the upregulation and downregulation of genes, respectively, after the ACK1 gene knockdown. Only DEGs (fold change ≥2 and adjusted P value <0.001) are colored.