Research Paper Volume 13, Issue 5 pp 6592—6605

Lupeol ameliorates LPS/D-GalN induced acute hepatic damage by suppressing inflammation and oxidative stress through TGFβ1-Nrf2 signal pathway

Lupeol attenuates liver injury induced by LPS in zebrafish. (A–D) Zebrafish larvae were treated with different concentrations of lupeol (0, 3.125, 6.25,12.5,25,50,100,200, and 400μmol) to observe changes in zebrafish morphology, survival rate, body length and heart rate. N=20, data are expressed as the mean ± SEM, *PE) Schematic diagram of treatment on zebrafish. (F) H&E staining of the liver in zebrafish larva. Magnification, 400×, bar=200μm.

Figure 1. Lupeol attenuates liver injury induced by LPS in zebrafish. (AD) Zebrafish larvae were treated with different concentrations of lupeol (0, 3.125, 6.25,12.5,25,50,100,200, and 400μmol) to observe changes in zebrafish morphology, survival rate, body length and heart rate. N=20, data are expressed as the mean ± SEM, *P<0.05, **P<0.01, ***P<0.001, control group vs administration group. (E) Schematic diagram of treatment on zebrafish. (F) H&E staining of the liver in zebrafish larva. Magnification, 400×, bar=200μm.