Figure 7. HNRNPU expression is upregulated in BRCA cell lines and clinical tumor samples. Altering expression of HNRNPU proteins in clinical tumor samples (A) and four human BRCA cell lines (B), as determined via western blot analysis. Glyceraldehyde 3-phosphate dehydrogenase was used as the control. ZR-75-1 and MDA-MB-330 cells were transfected with shRNA and the mRNA (C), and protein (D) expression levels of HNRNPU were measured via reverse transcription quantitative real-time polymerase chain reaction and western blotting. *P < 0.05; **P < 0.01; ***P < 0.001.