Research Paper Volume 13, Issue 4 pp 5020—5033

Toxic effect of titanium dioxide nanoparticles on corneas in vitro and in vivo

TiO2 NPs damaged corneas in vivo. (A) Images of eyeballs after indicated treatment in vivo, bar = 0.1 cm. (B) Histological observation of corneas with HE staining after TiO2 NPs treatment, bar = 20 μm. (C) Detection of apoptotic cells in cornea of TiO2 NPs-treated mice with TUNEL staining, bar = 20 μm. Measurement of MDA content (D), SOD activity (E) and GSH-Px activity (F) in vivo. *P

Figure 6. TiO2 NPs damaged corneas in vivo. (A) Images of eyeballs after indicated treatment in vivo, bar = 0.1 cm. (B) Histological observation of corneas with HE staining after TiO2 NPs treatment, bar = 20 μm. (C) Detection of apoptotic cells in cornea of TiO2 NPs-treated mice with TUNEL staining, bar = 20 μm. Measurement of MDA content (D), SOD activity (E) and GSH-Px activity (F) in vivo. *P<0.05, ***P<0.001.