Figure 3. CD68+ cells at calcification sites in the heart of dKO mice co-express RhoA. (A) Immunostaining of dKO heart sections (cardiac muscle at septum) with RhoA and CD68 antibodies showing that many of the accumulated CD68+ cells at the sites of ectopic calcification are RhoA+ (white arrows); whereas the CD68+ cells away from the calcification sites are RhoA- (dotted circle). (B) Immunostaining of dKO heart sections (interface of right ventricle and septum) with RhoA and CD68 antibodies showing accumulation of RhoA+/CD68+ cells at the endothelial barrier (cardiac endothelium, dotted lines) which are infiltrating through the endothelium from circulation towards the pathological area of the cardiac muscle (sub-image 2); whereas the CD68+ cells in the circulating blood are RhoA- (sub-image 3). N=6 for both WT and dKO mice (8-week old).