Research Paper Volume 13, Issue 3 pp 4522—4551

Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling

Hsa

Figure 7. Hsa_circ_0000199-miR-613/miR-206 axis participated in regulating autophagy of triple-negative breast cancer (TNBC) cells. (A) Beclin-1, LC3-II, p62 and Atg5 expressions were determined in MDA-MB-231 and MDA-MB-468 cell lines treated by NC, si-NC, si-hsa_circ_0000199 and si-hsa_circ_0000199+miR-206 inhibitor. **: P<0.01; ***: P<0.001. (B) Beclin-1, LC3-II, p62 and Atg5 expressions were detected among MDA-MB-231 and MDA-MB-468 cell lines of NC, si-NC, si-hsa_circ_0000199 and si-hsa_circ_0000199+miR-613 inhibitor groups. **: P<0.01; ***: P<0.001. (C) Monodansylcadaverine (MDC) fluorescence intensity was monitored among MDA-MB-231 and MDA-MB-468 cell lines transfected by NC, si-NC, si-hsa_circ_0000199 and si-hsa_circ_0000199+miR-613 inhibitor. (D) MDC fluorescence intensity of MDA-MB-231 and MDA-MB-468 cell lines was determined among NC, si-NC, si-hsa_circ_0000199 and si-hsa_circ_0000199+miR-206 inhibitor groups.