Research Paper Volume 13, Issue 3 pp 4617—4633

LNK promotes granulosa cell apoptosis in PCOS via negatively regulating insulin-stimulated AKT-FOXO3 pathway

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Figure 2.

Granulosa cell apoptosis and oocyte maturation rate in PCOS and control group. (A1) Comparison of early and late apoptosis rate of granulosa cells in PCOS and control group by Annexin V FITC-A flow cytometry analysis. Results are mean±SD.*p < 0.05 vs. data of control group. (A2, A3) Examples of the Annexin V FITC-A flow cytometry analysis results of control group (A2) and PCOS group (A3). n=8 for each group. (B) PCOS patients had a lower oocyte maturation rate (number of mature oocytes/total retrieved oocytes) than control group. Results are mean±SEM. *p < 0.05 vs. data of control group. (C) Pearson correlation analysis showed a positive correlation between LNK (relative mRNA level, fold of GAPDH) and apoptosis. (D) LNK (relative mRNA level, fold of GAPDH) was negatively correlated with oocyte maturation rate.