Research Paper Volume 13, Issue 3 pp 4634—4646

Identification of microRNA-16-5p and microRNA-21-5p in feces as potential noninvasive biomarkers for inflammatory bowel disease

MiR-21-5p and miR-16-5p are potential noninvasive feces markers for IBD. (A) Expression of miR-16-5p in fecal specimens from HC, UC, and CD. (B) Expression of miR-21-5p in fecal specimens from HC, UC, and CD. (C) Expression of seven candidate DEMs (let-7i-3p, miR-15a-5p, miR-16-5p, miR-21-5p, miR-326, miR-338-5p and miR-483-5p) in fecal specimens from HC, UC and CD. (D) Compared with normal intestinal mucosa tissue based on GSE101817, the expression of miR-21-5p in the intestinal mucosa of CD patients was up-regulated. (E) Diagnostic value of miR-16-5p and miR-21-5p in fecal specimens of IBD patients was evaluated by ROC curves. *p

Figure 1. MiR-21-5p and miR-16-5p are potential noninvasive feces markers for IBD. (A) Expression of miR-16-5p in fecal specimens from HC, UC, and CD. (B) Expression of miR-21-5p in fecal specimens from HC, UC, and CD. (C) Expression of seven candidate DEMs (let-7i-3p, miR-15a-5p, miR-16-5p, miR-21-5p, miR-326, miR-338-5p and miR-483-5p) in fecal specimens from HC, UC and CD. (D) Compared with normal intestinal mucosa tissue based on GSE101817, the expression of miR-21-5p in the intestinal mucosa of CD patients was up-regulated. (E) Diagnostic value of miR-16-5p and miR-21-5p in fecal specimens of IBD patients was evaluated by ROC curves. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; HC, healthy control; UC, ulcerative colitis; CD, Crohn’s disease; ns, not statistically significant; ROC, receiver operating characteristic.