Figure 4. Overexpression of Nampt rescued LPS-induced inhibition on osteogenic differentiation. On the 7th day, (A) ALP staining and (B) Measurement of ALP activity showing that ALP was increased in ad-Nampt cells. (C) Real-time PCR analysis showing Runx2, Col1a1 and OCN gene expression normalized to β-actin. (D) Western blot results showing the protein expression of Runx2, Col1a1 and OCN. The data are expressed as the mean ± SD. *, p<0.05; **, p<0.01. #, p<0.05 vs. con; ##, p<0.01 vs. con.