Research Paper Volume 13, Issue 4 pp 5164—5184

Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway

IMD alleviated AngII-induced AAA in mice by blocking Notch1 signaling. (A) Representative photographs of macroscopic features of AAA in male ApoE-/- mice. Control, saline infusion; AngII, AngII (1000 ng/kg/min) infusion; AngII+IMD (300 ng/kg/min) infusion; AngII+DAPT (10 mg/kg) gavage for 4 weeks. The arrows indicate typical AAA in ApoE-/- mice. Scale bars, 5 mm. (B) Incidence of AngII-induced AAA in animals and maximal abdominal aortic diameter of survivor mice at the end of 28 days. (C) Representative HE staining of aortas. Scale bar, 200 μm, 100 μm. (D) Representative VVG staining. Scale bar, 500 μm. (E) Representative in situ zymography of MMP activity in suprarenal aortas from ApoE-/- mice. Scale bar, 100 μm. Data are mean ± SD. *PP

Figure 3. IMD alleviated AngII-induced AAA in mice by blocking Notch1 signaling. (A) Representative photographs of macroscopic features of AAA in male ApoE-/- mice. Control, saline infusion; AngII, AngII (1000 ng/kg/min) infusion; AngII+IMD (300 ng/kg/min) infusion; AngII+DAPT (10 mg/kg) gavage for 4 weeks. The arrows indicate typical AAA in ApoE-/- mice. Scale bars, 5 mm. (B) Incidence of AngII-induced AAA in animals and maximal abdominal aortic diameter of survivor mice at the end of 28 days. (C) Representative HE staining of aortas. Scale bar, 200 μm, 100 μm. (D) Representative VVG staining. Scale bar, 500 μm. (E) Representative in situ zymography of MMP activity in suprarenal aortas from ApoE-/- mice. Scale bar, 100 μm. Data are mean ± SD. *P<0.05, **P<0.01 vs. Control. #P<0.05, ##P<0.01 vs. AngII.