Research Paper Volume 13, Issue 4 pp 5164—5184

Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway

IMD inhibited Notch1 signaling activation by ADAM10. (A) Quantitative real-time PCR analysis of mRNA levels of ADAM10 and ADAM17 in aortas of Control, AngII, AngII+IMD and AngII+DAPT in mice. (B) Western blot analysis of protein level of ADAM10 in aortas of Control, AngII, AngII+IMD and AngII+DAPT mice. Western blot analysis of protein levels of (C, D) NICD, the intracellular fragment of Notch1 and the full length Notch1 receptor and (E) ADAM10 and ADAM17 in AngII-induced macrophage cell line Raw264.7. n=3, Data are mean ± SD. *PP#P##PF) Western blot analysis of protein level of ADAM10 in AngII-induced macrophage cell line Raw264.7. Except for the first 3 groups, IMD receptor blocker IMD17-47 group was added. n=3, Data are mean ± SD. *P#P&PG) Western blot analysis of protein level of ADAM10 in AngII-induced macrophage cell line Raw264.7. Except for the first 3 groups, cAMP/PKA inhibitor H89, cGMP/PKG inhibitor Ly83583 and PI3K/Akt inhibitor LY294002 were added. n=3, Data are mean ± SD. *P#P$P

Figure 7. IMD inhibited Notch1 signaling activation by ADAM10. (A) Quantitative real-time PCR analysis of mRNA levels of ADAM10 and ADAM17 in aortas of Control, AngII, AngII+IMD and AngII+DAPT in mice. (B) Western blot analysis of protein level of ADAM10 in aortas of Control, AngII, AngII+IMD and AngII+DAPT mice. Western blot analysis of protein levels of (C, D) NICD, the intracellular fragment of Notch1 and the full length Notch1 receptor and (E) ADAM10 and ADAM17 in AngII-induced macrophage cell line Raw264.7. n=3, Data are mean ± SD. *P<0.05, **P<0.01 vs. Control. #P<0.05, ##P<0.01 vs. AngII. (F) Western blot analysis of protein level of ADAM10 in AngII-induced macrophage cell line Raw264.7. Except for the first 3 groups, IMD receptor blocker IMD17-47 group was added. n=3, Data are mean ± SD. *P<0.05 vs. Control. #P<0.05 vs. AngII. &P<0.05 vs. AngII+IMD. (G) Western blot analysis of protein level of ADAM10 in AngII-induced macrophage cell line Raw264.7. Except for the first 3 groups, cAMP/PKA inhibitor H89, cGMP/PKG inhibitor Ly83583 and PI3K/Akt inhibitor LY294002 were added. n=3, Data are mean ± SD. *P<0.05 vs. Control. #P<0.05 vs. AngII. $P<0.05 vs. AngII+IMD.