Research Paper Volume 13, Issue 4 pp 5185—5196

A short deletion in the DNA-binding domain of STAT3 suppresses growth and progression of colon cancer cells

STAT3del reduces in vitro growth, survival and migration of colon cancer cells. (A) CCK8 assay results show the proliferation rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3del. (B) TUNEL assay results show the apoptotic rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3del. (C) Wound healing assay results show the migration rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3del. * denotes p D, E) The top ten enriched GO terms related to biological process (D) and KEGG signaling pathways (E) based on the genes associated with differential peaks. (F) The mRNA and (G) protein levels of several genes with differential STAT3 binding ability in SW480 cells with wild type STAT3 or STAT3del. (H) STAT3 occupancy in genes with differential STAT3 binding ability in the SW480, SW620 and HCT116 cells with wild type STAT3 or STAT3del. * denotes p

Figure 3. STAT3del reduces in vitro growth, survival and migration of colon cancer cells. (A) CCK8 assay results show the proliferation rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3del. (B) TUNEL assay results show the apoptotic rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3del. (C) Wound healing assay results show the migration rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3del. * denotes p <0.05. Each experiment was performed with three replicates. (D, E) The top ten enriched GO terms related to biological process (D) and KEGG signaling pathways (E) based on the genes associated with differential peaks. (F) The mRNA and (G) protein levels of several genes with differential STAT3 binding ability in SW480 cells with wild type STAT3 or STAT3del. (H) STAT3 occupancy in genes with differential STAT3 binding ability in the SW480, SW620 and HCT116 cells with wild type STAT3 or STAT3del. * denotes p < 0.05. Each experiment was performed with three replicates.